[FMX] Separation and concentration of animal cell culture fluid by using anti-fouling membrane system

 

저자명: Hyun Woo Kim, Sung Teak Oh, Sang Ug Kim, Wuk Sang Ryu, Kyung Hee Jung, Eun Gyo Lee, Gi Teak Park
발행정보: 2017 KSBB Fall Meeting and International Symposium

❏ OBJECTIVES

- To minimize the impact on the disruption of mammalian cells and achieve effective separation and concentration

- To separate and concentrate large amounts of mammalian cells

- To achieve separation and concentration at a faster rate compared to the Depth filter


❏ INTRODUCTION

○ FMX

- Anti-fouling membrane system

- FMX system specialized in high-density, high-points and high-density mixtures

- Applying various membrane of MF, UF, and NF

○ Mammalian cell

- Mammalian cells to produce vaccines and therapeutic proteins

○ Membrane

- It consists of a polymer with different pores and is manufactured using a high manufacturing technique


❏ MATERIALS AND METHODS

○ Filtration

- FMX-miniB: BKT Co.,Ltd. (1 L working vol.) Membrane area 0.0033 m2, pump 0.75 L/min, blade 800 rpm, pressure 0.5bar

- Depth filter: Filtration Area 22 cm2 (3.41 in.2), pump 8 mL/min, poresize 8~20 ㎛)

FMX-miniB

FMX-miniB

FMX-B5

FMX-B5

Blade

Blade

○ Membranes (for FMX)

- AYTech 5 (5 ㎛), PTFE
- Donaldson 5 (5 ㎛), PTFE
- LG 015 (0.15 ㎛), PES

 
 

 ❏ RESULTS

○ Circulating operation FMX

Blade RPM & Flux Rate

Blade RPM & Flux Rate

Turbidity

Turbidity

Viability (%)

Viability (%)

- As time passes, the pore is clogged with reduced flux, slowly
- Flux increase after blade acceleration (13→36 ml/min)
- Turbidity increased by 2 → 4 NTU
- Maintained in viability of cells

Table 1. Comparison of Depth filter and FMX

Table 1. Comparison of Depth filter and FMX

 
Fig 1. The value of flux according to the variety of membrane(after centrifuge) (membrane – AYTech 5 ㎛, Donaldson 5 ㎛, LG 0.15 ㎛)

Fig 1. The value of flux according to the variety of membrane(after centrifuge)
(membrane – AYTech 5 ㎛, Donaldson 5 ㎛, LG 0.15 ㎛)

그림11.png
 
 

 ❏ CONCLUSIONS

- In the case of a filtration by FMX, the flux tends to increase as rpm increases

- Compared to the Depth filter, the FMX was able to filter with high quality within the appropriate turbidity below 10 NTU

- Optimum conditions were pump rate of 0.75 L/min, blade 800 rpm, and pressure 0.5 bar, the permeability of IgG was similar at depth filter

- Viability remained constant even with fast circulation rate and permeability of IgG exceeded more than 90 %., however, low flux values reduced throughput

- For further study to gain better flux value, screening and application of various membranes will be conducted

 
논문21 bktFMX